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KMID : 0352720090330010059
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Journal of Ginseng Research
2009 Volume.33 No. 1 p.59 ~ p.64
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Isolation and Characterization of Calmodulin Gene from Panax ginseng C. A. Meyer
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Wasnik Neha G.
Kim Yu-Jin
Kim Se-Hwa
S Sathymoorthy
Pulla Rama Krishna
Parvin Shohana
Senthil Kalaiselvi
Yang Deok-Chun
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Abstract
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Ca2+ and calmodulin (CaM), a key Ca2+ sensor in all eukaryotes, have been implicated for defense responses of plants. Eukaryotic CaM contains four structurally and functionally similar Ca2+ domains named Ⅰ, Ⅱ, Ⅲ and IV. Each Ca2+ binding loop consists of 12 amino acid residues with ligands arranged spatially to satisfy the octahedral symmetry of Ca2+ binding. To investigate the altered gene expression and the role of CaM in ginseng plant defense system, cDNA clone containing a CaM gene, designated PgCaM was isolated and sequenced from Panax ginseng. PgCaM, which has open reading frame of 450 nucleotides predicted to encode a precursor protein of 150 amino acid residues. Its sequence shows high homologies with a number of other CaMs, with more similarity to CaM of Daucus carota (AAQ63461). The expression of PgCaM in different P. ginseng organs was analyzed using real time PCR. The results showed that PgCaM expressed at different levels in young leaves, shoots, and roots of 3-week-old P. ginseng. In addition, the expressions of PgCaM under different abiotic stresses were analyzed at different time intervals.
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KEYWORD
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Abiotic stress, calmodulin, Panax ginseng, real-time PCR, stress
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